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Norsk språkHow to Calibrate in Image View?
Before you can make precise measurements with a measuring eyepiece or a microscope camera, you must first calibrate each device with a standard called an object micrometer. This calibration establishes the micrometer value, which indicates how large the distance is between two graduations of the micrometer on your specimen.
An object micrometer is a conventional slide that features a scale of 1 mm with 100 graduations. The distance between 2 divisions is, therefore, exactly 10 μm. For the micrometer calibration, the object micrometer is placed on the stage exactly like a specimen.
Because of the different magnifications of the objectives, you need to calibrate your microscope camera for each one individually, just like you do when calibrating a measuring eyepiece.
In this case, we’re using a microscope camera, software ImageView
1. Run ImageView;
2. Connect the camera to the computer and microscope;
3. Start the camera (Here, it is A59.2211);
4. Switch the microscope object to 10X and put the stage micrometer (calibration slide) in the middle of the microscope field and try to find the ruler clearly. Set the Unit to Pixel and the video Resolution to the maximum one (2048X1534 for A59.2211-3.1MPA) and Zoom ratio to 100%, see:
5. Choosing the Options>Calibrate••• command or clicking “
” on the toolbar, a red line with pixels number and 0.000um length is overlaid on the video window.
At the same time, a dialog called Calibrate will be displayed over the video window;
6. Try to align the two ends of the red line with the video ruler scale (Try to pull the red line as long as possible to keep the calibration accuracy);
7. Enter or select the current microscope objective lens Magnification in the Magnification field. The current Magnification is 10X;
8. Read the overlaid (by the red line) micro-ruler actual length and fill it in the Actual Length field, the current Actual Length is 450um. This Actual Length will also be displayed in the middle of the red line just behind the pixel number. At the same time, the Resolution will be calculated and displayed simultaneously in the Resolution field.
9. If everything is ok, click Ok to end the calibration. The Magnification 10X (This is the number you entered in the Magnification edit box) will be available in the Magnification dropdown combobox on the video window toolbar
10. If you wish to make the measurement under this Magnification, choose 10X in the above Magnification dropdown combobox first and then choose Unit in the Unit dropdown list box
which is just in the left of the Magnification’s dropdown combobox (User can also choose Unit through the Option>Measurements••• menu, a dialog called Measurement will be brought up.
Click Length Unit page to display the Length Unit page and and checking Unit in the current item.
Now, you can measure the objects with the selected Unit at ease.
11. The selected Resolution can be saved for future image measurement operations. If the measurements are performed on the video, the objects and resolution can be saved in the image with ImageView File Format (*.tft) for the future applications;
12. The other microscope magnifications such as 4X, 40X,100X can also be defined just as the above steps. Thus, when you switch the microscope objective lens, you just need to select the Magnification (for example)
on the toolbar to perform the measurement operations.
How to Measure in Image View?
The Measurements menu is mainly used for the image measurement. With this menu, you can measure the image with many geometrical shape at ease. The App uses Layer technique to manage the measurement object. This will never pollute the image pixels. The Measurements menus and its submenus are shown below.
Note: a) To check or modify the parameters of the selected Object, just select a single Object and the Measurement Sidebar will be activated and extended automatically. Here user can edit the corresponding Object parameters to modify its properties; b) The Appearance, Coordinates on Measurement Sidebar will be enabled only when a single Object is selected. The Calculation will be effective for a single selected Object or multiple selected Objects. Its calculated items will depend on the selected Object type (The type could be the same or different).
1, Object Select
The Measurements>Object Select menu or the toolbar button will be enabled only when a new Measurement is made over the Background Layer.
After the measurement is done on the specific layer, choose this menu to select the Objects.
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The Object can be selected by clicking on it;
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Select a group of Objects by including them in a rectangular area with Object Select command or by press down the Shift key and clicking the Object with left mouse button until all the desired Objects are selected.
2, Angle
2.1 Angle (3 Points)
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Move the mouse on the image to point 1, mark it by clicking the left mouse button;
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Move the mouse to point 2, mark it by clicking the left mouse button;
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Move the mouse to point 3, mark the final point by clicking the left mouse button. A label 50.26° will be shown near point 2.
2.2 Angle (4 Points)
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Move the mouse on the image to point 1, mark it by clicking the left mouse button;
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Move the mouse to point 2, mark it by clicking the left mouse button; Point 1 and point 2 will be connected automatically to form L12;
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Move the mouse to point 3, mark the first point of the second line;
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Move the mouse to point 4, mark it by clicking the left mouse button; Point 3 and point 4 will be connected automatically to form L34;
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After the above process, L12 and L34 will be extended to form an angle between L12 and L34.
A label 34.40° will be shown near vertex.
3, Point
Move mouse to the point; click the left mouse to mark it. It will show the point Label Ptn and its position x and y over the image.
4, Line
4.1 Line>Arbitrary Line
Draw Arbitrary Line between the two points on the specified layer.
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Choose Measurements>Line> Arbitrary Line command;
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Move mouse to the 1st point; click the left mouse button to mark it;
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Move mouse to the 2nd point, click the left mouse button to mark it again, a line with L1 and its length will be shown.
4.2 Line> Horizontal Line Ctrl+Left mouse button
Draw a Horizontal Line between the two points on the specified layer. When the 1st point is marked, the 2nd point’s y coordinate will be restricted to equal to the first point’s y coordinate automatically.
4.3 Line> Vertical Line Shift+Left mouse button
Draw a Vertical Line between the two points on the specified layer. When the first point is marked, the second point’s x coordinate will be restricted to equal to the first point’s x coordinate automatically.
4.4 Line>Multipoint Connection
Connect the left mouse button clicked points to form a straight line.
Ctrl+Left mouse button will draw a horizontal Multipoint Connection.
Shift+Left mouse button will draw a vertical Multipoint Connection.
5, Line(3 Points)
5.1 Line(3 Points)>Arbitrary Line
5.2 Line(3 Points)>Horizontal Line (Ctrl+Left mouse button)
5.3 Line(3 Points)>Vertical Line (Shift+Left mouse button)
All of these are just as Line>Arbitrary Line, Line>Horizontal Line Line>Vertical Line function, but the 3rd point is used to locate the label position.
6, Parallel
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Choose Measurements>Parallel command;
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Move mouse and click to mark the 1st point;
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Move mouse and click to mark the 2nd point;
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Move mouse and click to mark the 3rd point;
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Move mouse again, one will find the 4th point is always restricted to parallel to the line connecting point 1 and point 2. Click to mark the 4th point. Two parallel lines will be labeled and numbered.
7, Two Parallels
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Move mouse and click its left button to mark the 1st point;
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Move mouse and click its left button to mark the 2nd point. Line 1 (the 1st line) connect these two points will be overlaid on the image;
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Move mouse and click its left button to mark the 3rd point;
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Move mouse again, one will find the 4th point is always restricted to parallel to the Line 1 connecting point 1 and point 2. Click to mark the 4th point. Line 2 will be drawn which is parallel to Line 1. A dot line DL 1 located at the center of Line 1 and Line 2 will also be marked. Thus the 1st parallel is finished;
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Repeated step 1~4 will draw Line 3 and Line 4. A dot line DL 2 located between the center of Line 3 and Line 4 will also be marked. Thus the 2nd parallel is finished;
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The App will label the distance between DL1 and DL2 in with Unit specified in the Unit combo box. The final result is shown on the right side.
8, Vertical
8.1 Vertical>Four Points.
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Move mouse and click its left button to mark the 1st point;
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Move mouse and click its left button to mark the 2nd point. A line (the 1st line) connect these two point be overlaid on the image;
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Move mouse and click its left button to mark the 3rd point;
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Move mouse again, one will find the 4th point is always restricted to perpendicular to the line of point 1 and point 2. Click to mark the 4th point. Two vertical lines will be labeled and numbered.
8.2 Vertical>Three Points
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Move mouse and click its left button to mark the 1st point;
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Move mouse and click its left button to mark the 2nd point. A line (the 1st line) connecting these two points will be overlaid on the image;
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Move mouse and click its left button to mark the 3rd point. The 2nd line will be overlaid on the image which is perpendicular to the 1st one.
9, Rectangle
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Move mouse to the 1st point; click its left mouse button to mark it;
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Move mouse to the 2ed point; click its left mouse button to mark it. A Rectangle will be overlaid on the image according these two diagonal points.
10, Ellipse
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Choose Measurements>Ellipse command;
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Move mouse to mark the 1st point;
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Move mouse to mark the 2nd point;
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If it is not superposing with the shape on the image, choose Measurements>Object Select command to adjust the position to adjust the Ellipse in order to coincide with the image shape.
11, Circle
11.1 Circle>Center+Radius
Choose Measurements>Circle>Center+Radius command to draw a circle with Center+Radius method on the specified Layer. Here, the circle is [email protected].
11.2 Circle>Two Points
Choose Measurements>Circle>Two Points command to draw a circle with Two Points method on the specified layer.
11.3 Circle>Three Points
Choose Measurements>Circle>Three Points command to draw a circle with Three Points method on the specified layer.
12, Annulus
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Find the Annulus center 1 and click mouse button to mark the Annulus center;
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Move mouse to let the 1st circle superpose with the image circle, clicking the mouse left button;
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Move mouse to let the 2nd circle superpose the image circle, clicking the left mouse button. Two radiuses with number and units will be labeled on the Annulus.
13, Two Circles
13.1 Two Circle>Center+Radius(R)
Draw Two Circles with Center+Radius method on the specified layer. After the Two Circles are drawn, a line connecting these two circle centers will be drawn.
13.2 Two Circle>Three Points(3)
Draw Two Circles with Three Points method on the specified layer. After the Two Circles are drawn, a line connecting these two circle centers will be drawn.
14, Arc
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Move mouse to mark the 1st point;
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Move mouse to mark the 2nd point;
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Move mouse to mark the 3rd point, then an Arc with Label and its Length will be drawn connecting these three points.
15, Text
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Choose Measurements>Text command;
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Move mouse to mark the 1st point;
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Move mouse to mark the 2nd point, a rectangle with dash line restrict the Text window size. After the mouse button is released, a dialog called Text will bring up for you to enter the Text;
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Enter the Text and click the right mouse button to end the Text object.
Note: The Text, Text frame styles, and Text positions can be modified on the Measurement Sidebar.
16, Polygon
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Choose Measurements>Polygon command;
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Move mouse and click the left mouse button to mark the 1st point;
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Move mouse and click the left mouse button to mark the 2nd point;•••;
n. Move mouse and click the left mouse button to mark the nth point;
n+1. Click the right mouse button to end the Polygon command and the
nth point will be connected with the 1st point.
If only 2 points are marked and the 3rd action is the clicking of right mouse button, then nothing will be done.
17, Curve
17.1 Curve>Random Connection
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Press down the left mouse button and move mouse according to the requirement to draw any curve in Random Connection format;
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If the left mouse button is released and move the it to a new position and then click the left mouse button again will draw a line to connect the last point;
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Release the left mouse button and click the right mouse button to end Random Connection.
17.2 Curve>Multipoint Connection
The Model 2 Curve operation is almost the same as that of the Polygon.
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Choose Measurements>Curve> Multipoint Connection command;
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Move mouse to a point and click the left mouse button to mark the 1st point;
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Move mouse to a point and click the left mouse button to mark the 2nd point;
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•••;
n-1. Move mouse to a point and click the left mouse button to mark the (n-1)th point;
n. Move mouse to the final point and click the left mouse button to mark the nth point, then click the right mouse button to end the current Multipoint Connection process.
Choosing button can adjust the finished Multipoint Connection.
18, Scale Bar
Choose Measurements>Scale Bar command will bring up a Scale Bar dialog as shown on the right up figure.
Input the number in the length edit box to determine the Scale Bar length. The Unit is chosen according to the current Unit in the Options>Measurement>Length Unit page. If the Unit is pixel, then Scale Bar dialog will be change to the right bottom figure.
Change the default value (3000) to the desired length and directly click OK, a Scale Bar will be displayed on the image in measurement style.
One can move the Scale Bar to where ever he wants(Choosing Measurements>Object Select after the Scale Bar is displayed on the image/video window and move the mouse to the Scale Bar and click down the left
mouse button). The final result should be:
The Scale Bar can be rotated on the Measurement Sidebar as shown above.
19, Arrow
Draw an Arrow on the image to specify the key or interest point on the image or video.
20, Z Order
The Z Order submenu is shown above.
Top: Modify the selected Object'srelative position to the uppermost place of all Objects on the Current layer;
Bottom: Modify the selected Object's relative position to the lowest place of all Objects on the Current layer;
Move Up: Modify the selected Object's relative position to the higher place of the two on the Current layer;
Move Down: Modify the selected Object's relative position to the lower place of the two on the Current layer.
Calibration & Measure on Biological Microscope Video
Calibration & Measure on Stereo Microscope Video
How to Measure with Stage & Eyepiece Micrometer
Huang Xin