Fluorescent Microscope uses an imaging technique that allows the excitation of fluorophores and subsequent detection of the fluorescence signal. Fluorescence microscopes require a powerful light source (100W Mercury or 5W LED) and a filter cubes to dichroic mirror to reflect light at the desired excitation/emission wavelength. Fluorescence is produced when light excites or moves an electron to a higher energy state, immediately generating light of a longer wavelength, lower energy and different color to the original light absorbed. The filtered excitation light then passes through the objective to be focused onto the sample and the emitted light is filtered back onto the detector for image digitalization. It is widely used in biology and medicine, as well as in other fields.
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