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Automated Cryo-TEM Sample Freezing Solution A50.7098 is a grid plunge freezer that fast-fixes thin liquid films containing samples into vitrified ice layers using cryogens (such as liquid ethane). It prepares proteins, cells, and solution system materials for subsequent 3D Cryo-TEM analysis. |
Product Highlights |
•Highly automated, stable, and repeatable vitrification process. •Specifically designed for Cryo-TEM, compatible with various sample types. •Precise control of key parameters (4℃-60℃, humidity 40%-100%). | •Multi-physical stimulation functional integration. •Customization on demand. •Flexible adjustment of clamping and plunging parameters. |
User Interface User-friendly HMI design, easy to operate, suitable for various scenarios. | GlowDischargeUnit Recommended use with glow discharge unit for grid hydrophilization to achieve higher quality vitrified samples. |
Function This equipment can meet the sample preparation needs of various types of samples such as proteins, large particle suspensions, and viruses |
| NO. | Parameter | Specification |
| 1 | Humidity Control | Maximum humidity can reach 100% and remain stable. Capable of real-time monitoring of sample chamber humidity, with ultrasonic control function and control accuracy of ±1%. |
2 | Temperature Control | When the ambient temperature is between 18°C and 25°C,the chamber temperature can be controlled within the range of 4°C to 60°C, adjustable in increments of 0.5°C per step. |
| 3 | Sample Injection | Capable of preparing small-volume samples of 2–10 ul using a pipette for manual sample loading. |
| 4 | Sample Adsorption Method | Double-sided adsorption. |
| 5 | Sample Blotting Method | Blotting using filter paper. Blotting action and vitrification time are controllable. Users can customize blotting compensation and draining time via software. |
| 6 | Number of Blotting Cycles per Grid | ≥16 times, with multi‑adjustable blotting force level. Both the number of times and the force can be set via software. |
| 7 | Sample Loading Method | Manual touch screen control or foot pedal control. Optional: button control and linkage control. |
| 8 | Plunging Depth | Adjustable from 1 to 5 levels. |
| 9 | Blotting Control | All controllable via software; single blotting time is independently adjustable.Number of blotting actions is adjustable. |
| 10 | Application Time | Application time and waiting time can be controlled via software, with relevant parameters pre‑settable. |
| 11 | Secondary Coolant Reservoir Capacity | Dedicated container with <10 mL capacity. |
| 12 | Grid tweezers descent speed | <1 second. |
| 13 | Temperature control of coolant container and cryo-sample holder | Capable of synchronously lowering the temperature while keeping the grid submerged in the coolant. The coolant container can include an integrated anti‑contamination ring. |
| 14 | Method for transferring grids from coolant to grid box in liquid nitrogen environment | Semi‑automatic. Capable of transferring 4 or more grid boxes at one time. |
| 15 | Chamber lighting | The chamber is equipped with lighting for easy observation and operation; can be turned off. |
| 16 | Dewar Cup | The Dewar cup can be moved upward and can be used to pour out excess liquid nitrogen. |
| 17 | Shutter Operation | Equipped with automatic shutter control function. |
| 18 | Gripper Config | Equipped with a motor‑driven gripper to ensure highly repeatable clamping force. |
| 19 | Power Supply | 220V power supply, 300W power. |
Huang Xin