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Norsk språkHow to select OPTO-EDU Phase contrast microscope?
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What is Phase Contrast Microscope? A Phase Contrast microscope is a microscope used to observe unstained specimens. Living cells and unstained biological specimens, due to the difference in the refractive index and thickness of the fine structures of the cells, when the light wave passes through, the wavelength and amplitude do not change, only the phase changes (x phase difference). Unable to observe. The phase contrast microscope observes living cells and unstained specimens by changing this phase difference and using the diffraction and interference phenomena of light to convert the phase difference into amplitude difference. The difference between the phase contrast microscope and the ordinary microscope is that the iris diaphragm is replaced by a ring diaphragm, the ordinary objective lens is replaced by an objective lens with a phase plate, and a telescope for coaxial is provided. |
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Phase contrast is a light microscopy technique used to enhance the contrast of images of transparent and colourless specimens. It enables visualisation of cells and cell components that would be difficult to see using an ordinary light microscope. As phase contrast microscopy does not require cells to be killed, fixed or stained, the technique enables living cells, usually in culture, to be visualised in their natural state. This means biological processes can be seen and recorded at high contrast and specimen detail can be observed. Fluorescence staining can be used in combination with phase contrast to further improve the visualisation of samples. |
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Phase contrast is ideal for thinner samples, therefore an inverted microscope system can be used. This provides the additional advantage of having more working space. Phase contrast can also be installed on upright microscopes. If thicker samples need to be visualised in high-resolution, differential interference contrast (DIC) is a more suitable technique to use. |
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Components Needed for Phase Contrast It is relatively simple and inexpensive to adapt an inverted or upright light microscope for phase contrast. The following components need to be installed: ● Phase contrast condenser with a phase annulus (also known as a phase ring) ● Phase contrast objectives - a set of objectives which each contain a phase plate. ● Centering Telescope Eyepiece 10x There are 2 kinds of phase contrast condenser. The simple one has a socket for phase contrast slides, users need to exchange slides for different objectives during observation. It is a little bit complicated in operation, but users can choose 40x phase contrast objective & slide as a minimum set of phase contrast at a low cost, which is the most useful set in daily work. The turret phase contrast combines all four phase rings inside. It makes switch rings according to objectives easier, but the full set phase contrast attachment cost is higher. |
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Different Phast Contrast Method Negative Phase Contrast Applicable for the observation of the shape, quantity and living conditions of objects. Its background is little darker than Positive Phase Contrast. Positive Phase Contrast It is suitable for the observation of the internal microstructure of matter, but also for the observation of the shape, quantity and living conditions of objects. |
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DIC - Differential Interference Contrast It can not only observe colorless and transparent objects, but also the image presents a relief-like three-dimensional effect, and has some advantages that cannot be achieved by phase contrast microscopy, and the observation effect is more realistic. |
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How to Use Phase Contrast Microscope Step 1. Turn the turntable phase contrast condenser turntable to the "○" mark, and adjust the instrument referring to the bright field observation method. Put the phase contrast objective lens into the optical path, turn the phase contrast condenser turret, and turn the corresponding magnification scale to the front side. When the position is clearly felt, it means that the phase contrast ring plate has reached the desired position, and phase contrast observation can be performed |
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Step 2. Take observation with a 10× phase-contrast objective lens as an example. Put the 10× phase-contrast objective lens into the optical path, and push the corresponding hole of the insert plate to the position, or turn the turntable to the “10×” mark, and turn the aperture diaphragm to the maximum. Remove the eyepiece on one side and replace it with the centering eyepiece, loosen the fixing screw, and twitch the upper part of the eyepiece, and observe the field of view at the same time, so that the image of the phase contrast plate and phase contrast ring plate is clear. Then tighten the set screw |
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Step 3. Push the two adjustment handles into the adjustment hole to adjust the position, turn the two adjustment handles, adjust the position of the ring plate, and make the bright ring of the ring plate coincide with the dark ring of the phase plate. Remove the centering eyepiece and replace it with a flat-field eyepiece for phase contrast observation |
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How to Select a Phase Contrast Microscope Full Set Phase Contrast Microscope There are several models in set can be selected quickly: |
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A19.1008-40 Phase Contrast Microscope ● Phase Contrast Version Classical 107BN Biological Microscope ● Phase Contrast Objective 40x, Phase Contrast Condenser For 40x ● Centering Telescope 10x, Green Filter Included. PH 40x, 100x Optional ● Large Size Mechanical Stage 140x140mm, Moving Range 50x75mm ● High Brightness 3W LED Light Source, Brightness Adjustable |
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A19.0209-40 Phase Contrast Microscope ● Sliding Binocular Head 30° Inclined, 360° Rotatable ● Achromatic 4x,10x,100x, Achromatic Phase Contrast 40x Objective ● Double Layer Mechanical Stage, 140x140mm, Mvoing Range 5075mm ● Phase Contrast Slide For 40x, Phase Contrast Condenser, Centering Telescope 10x ● Built-in Illumination 6V20W Halogen Lamp, 100~240V |
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A19.0214 Phase Contrast Microscope, Turret Phase Contrast ● Sedentopf Trinocular Head 30° Inclined 360° Rotatable ● Achromatic Phase Contrast Objective 10x20x40x100x ● Turret Disc Phase Contrast Condenser, Centering Telescope ● Large Size Double Layer Mechanical Stage 160x140mm/75x50mm ● 6V20W Halogen Lamp Kohler Illumination |
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A19.2702-T Phase Contrast Microscope, Infinity Plan, Turret Disc Condenser ● UCIS Infinity Plan Achromatic Phase Contrast Optical System ● WF10X/22mm High Eye-Point Eyepiece, Centering Telescope For PH ● Infinity Plan Phase Contrast Objective 10x20x40x100x ● Turret Disc Phase Contrast Condenser For 10x20x40x100x Objective ● Double Layer Mechanical 156x138mm, Kohler 3W LED Illumination |
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A19.0911 Phase Contrast Microscope ● Infinity Chromatic Aberration Correction System, Bright Field & Phase Contrast ● Infinity Plan Phase Contrast Objective 10x20x40x100x & Centering Telescope 10x ● Turret Phase Contrast Condenser, For 10x20x40x100x Phase Contrast ● Double Layer Mechanical Stage 150x162mm /76×50mm ● 5 Holes Coded Nosepiece Enable Brightness Memory Function On LCD Information Screen Lab Microscope + Phase Contrast Attachment Most of A12. Series laboratory microscopes have optional Phase Contrast attachment. You can choose them freely according to your needs and budget. Here are some popular models for reference: |
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A12.2603 Biological Microscope Inverted Phase Contrast Microscope Most inverted microscopes have phase contrast attachments included, so these microscopes can be called Phase Contrast microscopes too! Below picture shows light path & codenser annulus in inverted microscope. |
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Here are some popular models from Opto-Edu for reference: |
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● Seidentopf Trinocular Head, Light Split Switch E100:P0 / E20:P80 ● LWD Infinity Plan Semi-APO Fluorescent Objectives Bright Field & Phase Contrast Observation Supported ● LWD Condenser N.A.0.3 Working Distance 72mm, Up To 195mm Without Condenser ● Standard Halogen 6V30W Koehler Illumination, 5W LED Optional For Choice ● Fluorescence Attachment Can Be Added To Upgrade It As A16.2614 Fluorescent Microscope A14.2603 model customized for a professional and comfortable working environment Infinite distance color correction optical system, with the international advanced level of the new generation of products, A14.2603 for cell tissue observation and culture, the ideal partner for life science research |
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● Dual Photo & Video Channel Support Eyepiece / Digital Camera / SLR Camera View At Same Time ● ECO Automatic Power Off Upon User Leaving 10 Minutes & Power On Upon User Back ● Semi-APO LWD Infinity Plan Objectives Bright Field & Phase Contrast Observation Supported ● 4 Holes Disc Phase Contrast Condenser + Halogen 12V50W Koehler Illumination ● Fluorescence Attachment Can Be Added Upgrade To A16.2614 Fluorescent Microscope |
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A14.0205 Inverted Biological Phase Contrast Microscope, DIC, PMC ● Inverted Biological Phase Contrast Microscope, Upgradeable To DIC, PMC ● WF10x/22 Eyepiece, Bright Field LWD Infinity Plan 10x20x40x, ● Large Working Stage 224x205mm, Moving Stage 112x77mm ● LWD Condenser W.D.70mm With Phase Contrast Slide ● LWD Infinity Plan Phase Contrast Objective, Hoffman Objective, DIC Objective A14.0205 uses a streamlined design concept, compact and stable high rigid body, fully reflects the microoperation vibration prevention requirements. The ultra-long working distance light concentrating system can observe the culture cells without contamination in the high petri dish or cylindric flask. The lighting system fully considers heat dissipation and safety, and the ergonomic design concept, so that the operation is more convenient, comfortable and safe, and the space is wider. A14.0205 can be used for microscopic observation of cell tissue, transparent liquid tissue, also can be used for dynamic microscopic observation of culture tissue in the petri dish, can be applied to scientific research institutes, colleges and universities, medical health, inspection and quarantine, agriculture and dairy industry and other departments. |
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For any further questions or inquires about phase contrast microscope, you are welcome to contact us at [email protected]. We will be happy to assist in selecting the Best Suit model! Written By Shirley, 2022-12 |
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Huang Xin